Extended Data Fig. 4: Arginine mutant of Siglec -7 -9 and hCD22.
From: Measuring carbohydrate recognition profile of lectins on live cells using liquid glycan array (LiGA)
Data mirrored from Fig. 3for better visualization. a) Visual representation of glycans in the library. b) Siglec-7 R124A showed a lower enrichment compared to the wild-type Siglec on CHO cells binding, n = 10 test and n = 15 control samples. c) Siglec-9 R120A binding to LiGA, n = 10 test and n = 15 control samples. d) hCD22 R120A mutant showed a lack of binding, n = 5 test and n = 15 control samples. All respective n values are independent biological replicates. In b–d, the FC was calculated by Bioconductor edgeR DE analysis using the negative binomial model, TMM normalization, and BH correction for FDR (n= 5 for each cell type). Error bars represent s.d. propagated from the variance of the TMM-normalized sequencing data.
