Extended Data Fig. 1: Order of sample loading for antibody screening in WB and IP-WB.

a) A scanned Ponceau S-stained membrane used for antibody screening in WB. Master mixes of MWM, WT and KO lysates were prepared, and samples were loaded in the following order on a 12-well SDS–PAGE gel: MWM, WT and KO lysates. Commercial 12-well gels have two side wells. The right side well was used here for MWM (+). Up to 4 antibodies can be tested in WB on a single 12-well gel. b) Scanned Ponceau S-stained membrane used for antibody screening in IP. Samples are loaded in the following order on a 12-wells SDS–PAGE gel. Up to 3 antibodies can be tested in IP on a single 12-well gel. Transfers from 4-20% TG gels are shown as examples in a) and b). MWM=molecular weight marker, SM=starting material, UB=unbound fraction, IP=immunoprecipitate.