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Byproducts in the synthesis of [68Ga]Ga-PSMA-11

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arising from M. E. Rodnick et al. Nature Protocols https://doi.org/10.1038/s41596-021-00662-7 (2022)

Gallium-68-labeled HBED-CC-PSMA-11 ([68Ga]Ga-PSMA-11) is a well-known radiotracer widely used for the early detection of prostate cancer1,2. Of the various protocols for the clinical production of [68Ga]Ga-PSMA-11 that have been reported, the protocol presented by Rodnick et al. is particularly useful as it outlines the entire production process, including safety details and quality control, using either generator-derived 68Ga (pathway 1) or cyclotron-produced 68Ga (pathway 2)3. Given the widespread availability of 68Ga generators, pathway 1 would be the choice for many clinics and hospitals.

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Fig. 1: Thermally induced lactamization of PSMA conjugates4.
Fig. 2: HPLC chromatogram of natGa-labeled HBED-CC-PSMA-11.

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Acknowledgements

This work was partially supported by the Deutsche Forschungsgemeinschaft project number 546563412. We would also like to acknowledge W. Maison for his support.

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Authors and Affiliations

  1. Institute of Pharmacy, University of Hamburg, Hamburg, Germany

    Ata Makarem  (عطا مکارم)

  2. College of Chemical Engineering, Nanjing Forestry University, Nanjing, China

    Jianlin Han

  3. Molecular Structure Analysis, German Cancer Research Center, Heidelberg, Germany

    Karel D. Klika

Authors
  1. Ata Makarem  (عطا مکارم)
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  2. Jianlin Han
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  3. Karel D. Klika
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Contributions

A.M. coordinated and directed the work and wrote the manuscript. J.H. served as the scientific advisor and reviewed the manuscript. K.D.K. served as the scientific advisor and contributed to writing, reviewing and editing the manuscript.

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Correspondence to Ata Makarem  (عطا مکارم).

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Nature Protocols thanks Mark Daniel Bartholomä and Joseph Ioppolo for their contribution to the peer review of this work.

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Makarem, A., Han, J. & Klika, K.D. Byproducts in the synthesis of [68Ga]Ga-PSMA-11. Nat Protoc (2025). https://doi.org/10.1038/s41596-025-01164-6

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