Extended Data Fig. 3: Assessment of cDNA and library quality.
From: Single-nucleus total RNA sequencing of formalin-fixed paraffin-embedded samples using snRandom-seq
a, Example of a qPCR amplification curve showing the expected cycle threshold for high-quality extension products (11 cycles, indicated by the flat line crossing the fluorescence intensity curve). b, cDNA fragment size distribution analysis using a Qsep100 bio-fragment analyzer. The overall size distribution should range between 100 and 1,000 bp, with minimal content in the 100–200-bp range. c, Library fragment size distribution analysis using a Qsep100 bio-fragment analyzer. The analysis should show a single dominant peak with fragments predominantly between 300 and 800 bp. LM, lower marker (20 bp); UM, upper marker (1,000 bp).
