Extended Data Fig. 2: Functional identification of Liver Organoid.
a. Expression of hepatocyte genes was determined by qPCR after ProliHHs maturation induction. Data are normalized to freshly thawed PHH (n =5). The data were shown as the mean ± SD. b. Co-immunofluorescent staining of CYP1A2 and CYP3A4 in ProliHH-derived liver organoids. Scale bars, 100 mm. c. Co-immunofluorescent staining of E-Cadherin and multidrug resistance-associated protein 2 in ProliHH-derived liver organoids. Scale bars, 100 mm. d. Immunofluorescent staining of Ki67 in ProliHH-derived liver organoids. Scale bars, 100 mm. e. Expression of progenitor-associated genes was determined by qPCR after ProliHHs maturation induction (n = 5). Data are normalized to freshly thawed PHH and the data were shown as the mean ± SD. f. Immunofluorescent staining of EPCAM in ProliHH-derived liver organoids. Scale bars, 100 mm. g. Expression of AFP was determined by qPCR after ProliHHs maturation induction (n = 3), the data were shown as the mean ± SD. Data provide the amplification cycles of the AFP gene, using PHH and HepG2 cells as negative and positive controls for AFP expression, respectively. h. Representative time-lapse images of ProliHH-derived liver organoids from a healthy donor demonstrating the uptake of pHrodo™ Green-LDL.
