Extended Data Fig. 3: ProliHH cryopreservation and resuscitation.
a. Representative images of Trypan Blue staining and live/dead staining of resuscitated ProliHH after cryopreservation b. The percentage of live cells of resuscitated ProliHH as determined by Trypan Blue exclusion assay (n = 3), the data were shown as the mean ± SD. c. Time-lapse images of resuscitated ProliHH cultivation. d. Relative cell proliferation (fold change) of ProliHHs in normal (n = 3) and resuscitated ProliHH (n = 5), the data were shown as the mean ± SD. e. Expression of hepatocyte genes and progenitor-associated genes in normal and resuscitated ProliHH cells was determined by qPCR (n = 4), the data were shown as the mean ± SD.
