Correction to: Nature Protocols https://doi.org/10.1038/s41596-024-01014-x, published online 2 July 2024.

In the version of this article originally published, there were typographical errors in the Materials: Reagents list, where “vGlut1 antibody for vesicle isolation (SYSY, cat. no. 135 311, RRID:AB_887880)” now replaces “vGlut1 antibody for vesicle isolation (SYSY, cat. no. 135 011; RRID: AB_2617087),” where “Sepharose CL-4B (Millipore Sigma, cat. no GE17-0150-01” now replaces “Sepharose CL-4B (Millipore Sigma, cat. no. GE17-01501-01),” where “1-oleoyl-2-2(12-biotinyl(aminododecanoyl))-sn-glycero-3-phosphoethanolamine (sodium salt)” now replaces “1,2-Dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(cap biotinyl) (sodium salt),” and where “RRID: AB_2198854” has been now been added to “Synaptophysin mouse monoclonal antibody for western blot (Abcam, cat. no. ab8049; RRID: AB_2198854).”. Furthermore, “mPEG-SVA (molecular weight: 5,000 daltons; Laysan Bio, cat. no. mPEG-SVA-5000)” has been added before “Biotin-PEG-succinimidyl valerate (molecular weight: 5,000 daltons; Laysan Bio, cat. no. biotin-PEG-SVA-5000)”. The reagents have been updated in the HTML and PDF sections of the article.

For clarity, “At variance to ref. 20 here we used Biotin-PEG-SVA and mPEG-SVA since the SCM compounds used in ref. 20 are not available anymore from the vendor.” has been added in Step 33.

Finally, there are several instances where our previous protocol was mistakenly cited as reference 1. These have been corrected to ref. 20 and adjacent references renumbered accordingly.