Abstract
A new series of 6-hydroxychromone-based thiosemicarbazones 4(a-p) was synthesized and assessed for their antidiabetic (α-Glucosidase and α-Amylase inhibition) as well as antioxidant (2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2´-azinobis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS)) activities. Among the synthesized compounds, compound 4k (IC50 = 1.18 ± 0.19 µg/mL) emerged as the promising α-Glucosidase inhibitor, significantly outperforming the reference drug Acarbose (IC50 = 7.33 ± 0.13 µg/mL). For α-Amylase inhibition, compound 4 g (IC50 = 13.61 ± 2.04 µg/mL) demonstrated excellent activity, compared to Acarbose (IC50 = 43.15 ± 5.22 µg/mL). In antioxidant assays, compound 4o (IC50 = 15.30 ± 1.70 µg/mL) exhibited the strongest DPPH radical scavenging effect, and compound 4 g (IC50 = 6.06 ± 0.15 µg/mL) showed the highest ABTS scavenging activity, surpassing the standard antioxidant Trolox (IC50 = 30.20 ± 5.14 & 18.19 ± 2.47 µg/mL, respectively). Remarkably, these derivatives showed greater efficacy compared to standard inhibitors, underscoring their promise as novel candidates for antidiabetic and antioxidant drug development. Molecular docking analysis demonstrated strong binding and critical interactions within the enzyme active sites. MD simulations confirmed the stability of 4k-α-Glucosidase and 4 g-α-Amylase, with RMSD values below 3.6 Å, low RMSF (< 2.8 Å) at the binding site, and sustained key interactions with Phe 158 and Tyr 151, respectively. The network pharmacology further supported the findings of molecular docking and simulation analysis.
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All data generated or analyzed during this study are included in this published article [and its supplementary information files.
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Acknowledgements
The authors express their appreciation to the Deanship of Scientific Research at King Khalid University, Saudi Arabia, for this work through Large Research Project under grant number RGP-2/691/46.
Funding
The authors express their appreciation to the Deanship of Scientific Research at King Khalid University, Saudi Arabia, for this work through Large Research Project under grant number RGP-2/691/46.
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Wajeeha Zareen, Farhan Siddique: Investigation, Formal analysis. Nadeem Ahmed : Writing – original draft, Validation. Mostafa A. Ismail, Rima D. Alharthy: Formal analysis, software, Funding acquisition. Parham Taslimi, Mariya al-Rashida, Talha Islam: Formal analysis, software, Data curation, Investigation. Supervision, Conceptualization. Magdi E. A. Zaki, Sobhi M. Gomha: Formal analysis, Data curation, Funding acquisition. Zahid Shafiq, Ali Muhamad Khan : Writing – original draft, Supervision, Conceptualization.
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Zareen, W., Ahmed, N., Siddique, F. et al. Synthesis and biological evaluation of 6-hydroxychromone based thiosemicarbazones as potential antidiabetic and antioxidant agents. Sci Rep (2026). https://doi.org/10.1038/s41598-026-40449-y
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DOI: https://doi.org/10.1038/s41598-026-40449-y
