Extended Data Fig. 1: Maximal signalling and potency of the 47 GIPR variants in cAMP production, and the relation between WT GIPR gene expression and signalling.
a, cAMP production Emax, shown as percentage of WT GIPR activity. The variants are arranged according to the cAMP production (efficacy at 100 pM GIP). Grey, WT-like; dark red, below 50% activity. b, cAMP production potency (logEC50). c, Emax (cAMP production) vs Bmax (homologues competitive binding) of WT GIPR, expressed at DNA doses ranging from 10 ng to 10 µg. d, GIP binding affinity (Kd) of WT GIPR at DNA doses ranging from 10 ng to 10 µg. e, Potency (logEC50) of WT GIPR at DNA doses ranging from 10 ng to 10 µg in cAMP production. f, Linear correlation between WT GIPR expression and signalling of the 47 GIPR variants. Expression was measured as Bmax, and signalling was measured as cAMP production efficacy at 100 pM. The data represent the mean ± s.e.m. of a minimum of N = 3. Independent experiments were performed in duplicate.
