Extended Data Fig. 9: Genetic incorporation of Kbhb at Aldob K108.
From: Ketogenic diet reshapes cancer metabolism through lysine β-hydroxybutyrylation
a Immunoblotting and coomassie brilliant blue staining analysis of purified Aldob WT and K108bhb. b Intact mass measurement by MALDI-TOF verified the successful introduction of protein β-hydroxybutyrylation at K108 of Aldob. Calculated mass: 43205. c MS/MS analysis confirmed the successful incorporation of Kbhb at Aldob K108. d Enzymatic activities of purified Aldob WT, Aldob mutants, Aldob-K108bhb and Aldob-K108ac. The enzymatic activity of Aldob was measured by monitoring the consumption of NADH. e Relative enzyme activity determined at 14 minutes in (d). Similar results were obtained in three independent experiments (a, d). Statistical data are presented as mean ± SEM (e), P values were determined by one-way ANOVA, followed by Dunnett’s test (e).
