Extended Data Fig. 7: SSP inhibition suppresses de novo serine biosynthesis.
From: The unique catalytic properties of PSAT1 mediate metabolic adaptation to glutamine blockade
a, [U-13C6]-glucose stable-isotope tracing data showing the effect of 16 h treatment with 12 μM NCT-503 on de novo synthesized serine (m + 3) in parental and CB839RS MDA-MB-231 cells. Data are presented as mean values ± SD, n = 4 biological replicates. Schematic shows incorporation of labeled carbons from glucose into serine and glycine via the SSP. b, [U-13C6]-glucose stable-isotope tracing data showing the effect of 16 h treatment with 10 μM BI-4916 on de novo synthesized serine (m + 3) in parental and GlnIND cells. Data are presented as mean values ± SD, n = 3 biological replicates. CB839RS, CB-839 resistant derivative; GlnIND, glutamine-independent; 3-PG, 3-phosphoglycerate; PHGDH, D-3-phosphoglycerate dehydrogenase.
