Extended Data Fig. 2: NEAA dropout analysis.
From: The unique catalytic properties of PSAT1 mediate metabolic adaptation to glutamine blockade
a, Sensitivity of BT-549 cells to 6 days deprivation of individual NEAAs. Cells were cultured in complete media (parental) or glutamine-free media (GlnIND) lacking individual NEAAs. Viable cell counts under control conditions were set as 100%. Red triangle, dependence unique to GlnIND cells. Green triangle, dependence unique to parental cells. Data are presented as mean values ± SD, n = 3 biological replicates. Two-tailed unpaired t-test. * P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.001. b, NEAA sensitivity analysis as in panel ‘a’, but with MDA-MB-231 cells cultured in complete media (parental) or complete media supplemented with 500 nM CB-839 (CB839RS). Data are presented as mean values ± SD, n = 3 biological replicates. Two-tailed unpaired t-test. * P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.001. Parental MDA-MB-231 data are shared between Fig. 1i and Extended Data Fig. 2b for ease of comparison. CB839RS, CB-839 resistant; NA, not applicable; NEAA, nonessential amino acids.
