Extended Data Fig. 2: ACLY loss in Brown Fat Causes Tissue Whitening and Cold Intolerance in Female Mice.

a. Representative H&E of the iBAT from Acly^BATKO female mouse and littermate control. Scale bar 50um. Clear consistent phenotype across n=3 mice. b. Body weight of the Acly^BATKO female mice. n=7 C, 11 KO. c. Adipose tissue weights of the Acly^BATKO female mice. *p= 0.0232. n=7 C, 11 KO. d. Lean tissue weights of the Acly^BATKO female mice. n=7 C, 11 KO. e. Rectal temperatures of the Acly^BATKO female mice and littermate controls during cold exposure (4 °C). n=8 C, 8 KO. f. ‘Survival curve’, how many mice maintain body temperature above 30 °C, remain in the cold over the time course of experiment. n=8 C, 8 KO. g. Representative H&E of the iBAT from Acss2^BATKO female mouse and littermate control. Scale bar 50um. Consistent across n=6 mice. h. Body weight of the Acss2^BATKO female mice. n=5 C, 6 KO. i. Adipose tissue weights of the Acss2^BATKO female mice. n=5 C, 6 KO. j. Lean tissue weights of the Acss2^BATKO female mice. n=5 C, 6 KO. k. Rectal temperatures of the Acss2^BATKO female mice and littermate controls during cold exposure (4 °C). n=4 C, 5 KO. l. ‘Survival curve’, how many mice maintain body temperature above 30 °C, remain in the cold over the time course of experiment. n=4 C, 5 KO. Data are mean ± s.e.m. Statistical analysis unpaired two-tailed Student’s t-test.