Extended Data Fig. 3: BAT ACLY Loss Broadly Impairs Metabolism. | Nature Metabolism

Extended Data Fig. 3: BAT ACLY Loss Broadly Impairs Metabolism.

From: Brown fat ATP-citrate lyase links carbohydrate availability to thermogenesis and guards against metabolic stress

Extended Data Fig. 3

a. Lipidomics analysis of DAG in the AclyBATKO mice BAT. ***p= 0.0007. n=9 C, 14 KO. b. Lipidomics analysis of TAG in the AclyBATKO mice BAT. n=9 C, 14 KO. c. Lipidomics analysis of CE in the AclyBATKO mice BAT. **p= 0.0035. n=9 C, 14 KO. d. Lipidomics analysis of DAG degree of saturation in the AclyBATKO mice BAT. ***p= 0.0001, **p= 0.0002. n=9 C, 14 KO. e. Lipidomics analysis of TAG degree of saturation in the AclyBATKO mice BAT. **** <0.0001, ***p= 0.0001, ***p= 0.0002. n=9 C, 14 KO. f. D2O labeling of newly synthesized palmitate in the AclyBATKO mice BAT and their littermates. *p= 0.0163. n=8 C, 8 KO. g. Palmitate abundance in BAT of AclyBATKO mice and littermate controls. **p= 0.0087. n=8 C, 8 KO. h. D2O enrichment in plasma of AclyBATKO mice and littermate controls. n=8 C, 8 KO. i. 3H-2-deoxy-glucose uptake assay into interscapular BAT, subscapular BAT, SAT, Quad and Liver of the AclyBATKO mice after 30 min at 4 °C. ***p= 0.0003, ***p= 0.0004. n=4 C, 5 KO. j. RT-PCR analysis of Glut1 and Glut4 in the AclyBATKO mice BAT. **p= 0.0015. n=6 C, 6 KO. k. RT-PCR analysis of cd36 and lpl in the AclyBATKO mice BAT. ***p= 0.0001, **p= 0.0072. n=6 C, 6 KO. Panels A-K: grey bar (C), orange bar (AclyBATKO). Data are mean ± s.e.m. Statistical analysis unpaired two-tailed Student’s t-test.

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