Extended Data Fig. 4: The Thermogenic Gene Program Requires ACLY. | Nature Metabolism

Extended Data Fig. 4: The Thermogenic Gene Program Requires ACLY.

From: Brown fat ATP-citrate lyase links carbohydrate availability to thermogenesis and guards against metabolic stress

Extended Data Fig. 4

a. Western blot of siACLY treated brown mature adipocytes. n=2 samples, result has been replicated. b. Seahorse Mito Stress Test OCR measurements of siACLY treated brown mature adipocytes. ****p <0.0001. n=30NT, n=30 siACLY wells, result has been replicated. c. Seahorse Mito Stress Test ECAR measurements of siACLY treated brown mature adipocytes. ****p <0.0001. n=30NT, n=30 siACLY wells, result has been replicated. d. Mitochondria/Nuclear DNA ratio in the siACLY treated brown mature adipocytes. n=6 C, 6 KO. e. RT-PCR analysis of Acly, Ucp1, Cycs and Pgc1a in the siRNA treated brown mature adipocytes.*p=0.0251, ***p= 0.0005, *p=0.0004, ***p=0.0373. n=4 C, 4 KO. f. Western blot of siACLY treated brown mature adipocytes, chromatin and WCL (whole cell lysate). n=3 samples. g. Western blot of ACLY inhibitor (BMS-303141) treated brown mature adipocytes, chromatin and WCL (whole cell lysate). n=2 samples. Panels B-E: grey bar (NT), orange bar (siAcly). Panels B, C: black line (NT), orange line (siAcly). Data are mean ± s.e.m. Statistical analysis unpaired two-tailed Student’s t-test.

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