Extended Data Fig. 8: Fasn^BATKO male and female mice.

a. Representative H&E of the iBAT from Fasn^BATKO male and female mouse and littermate control. Scale bar 50um. Has been confirmed in n=3 males, n=5 females. b. Adipose tissue weights of the Fasn^BATKO male mice. n=6 C, 6 KO. Adipose tissue weights of the Fasn^BATKO female mice. n=6 C, 6 KO. c. Western blot of the Fasn^BATKO male and female mice. d. Rectal temperatures of the Fasn^BATKO male mice and littermate controls during cold exposure (4 °C). n=7 C, 7 KO. Rectal temperatures of the Fasn^BATKO female mice and littermate controls during cold exposure (4 °C). n=8 C, 6 KO. e. Mitochondria/Nuclear DNA ratio in the Fasn^BATKO mice BAT. n=6 C, 6 KO. f. CoQ levels in the Fasn^BATKO mice BAT. n=9 C, 8 KO. Panels B, E, F: grey bars (C), blue bars (Fasn^BATKO). Panel D: black lines (C), blue lines (Fasn^BATKO). Data are mean ± s.e.m. Statistical analysis unpaired two-tailed Student’s t-test.

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