Extended Data Fig. 9: Acly,Acss2BATDKO male and female mice. | Nature Metabolism

Extended Data Fig. 9: Acly,Acss2BATDKO male and female mice.

From: Brown fat ATP-citrate lyase links carbohydrate availability to thermogenesis and guards against metabolic stress

Extended Data Fig. 9

a. Representative H&E of the iBAT from Acly,Acss2BATKO male mouse and littermate control. Scale bar 50um. Clear consistent phenotype, confirmed n=6 mice. b. Western blot of the Acly,Acss2BATKO male mouse BAT. c. Adipose tissue weights of the Acly,Acss2BATKO male mice. *p=0.0291. n=5 C, 6 KO. d. Lean tissue weights of the Acly,Acss2BATKO male mice. n=5 C, 6 KO. e. Body weight of the Acly,Acss2BATKO male mice. n=5 C, 6 KO. f. Rectal temperatures of the Acly,Acss2BATKO male mice and littermate controls during cold exposure (4 °C), left. ‘Survival curve’, how many mice remain in the cold over the time course of experiment, right. n=7 C, 8 KO. g. Mitochondria/Nuclear DNA ratio in the Acly,Acss2BATKO male mice BAT. **p=0.0060. n=5 C, 6 KO. h. Representative H&E of the iBAT from Acly,Acss2BATKO female mouse and littermate control. Scale bar 50um. Clear consistent phenotype, confirmed in n=4 mice. i. Adipose tissue weights of the Acly,Acss2BATKO female mice. n=6 C, 5 KO. j. Lean tissue weights of the Acly,Acss2BATKO female mice. n=6 C, 5 KO. k. Body weight of the Acly, Acss2BATKO female mice. n=6 C, 5 KO. Panels C-E,G: grey bars (C), teal (Acly,Acss2BATKO) males. Panels I-K: grey bars (C), blue bars (Acly,Acss2BATKO) females. Panels F: black lines (C), teal lines (Acly,Acss2BATKO). Data are mean ± s.e.m. Statistical analysis unpaired two-tailed Student’s t-test.

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