Extended Data Fig. 10: BAT Acly;Fasn Double Knockout Rescues Thermogenesis Independent of DNL and Prevents ISR. | Nature Metabolism

Extended Data Fig. 10: BAT Acly;Fasn Double Knockout Rescues Thermogenesis Independent of DNL and Prevents ISR.

From: Brown fat ATP-citrate lyase links carbohydrate availability to thermogenesis and guards against metabolic stress

Extended Data Fig. 10

a. Body weight of the Acly,FasnBATKO male mice. n=5 C, 5 KO. b. Adipose tissue weights of the Acly,FasnBATKO male mice. n=5 C, 5 KO. c. Relative labeled abundance of palmitate in BAT of AclyBATKO,Acss2BATKO(n=11C, 5KO and 5KO), FasnBATKO(n=6C, 6KO),Acly/FasnBATKO(n=6C, 6KO)mice and littermate controls. *p=0.0404, ***p= 0.0007, **p=0.0035. d. Representative H&E of the iBAT from AclyBATiKO and Acly,FasnBATiKO male mouse and littermate control. Scale bar 50um. Has been confirmed in n=3 mice each. e. Body weight of the Acly,FasnBATiKO male mice. n=6 C, 6 KO. f. Adipose tissue and lean tissues weights of the Acly,FasnBATiKO male mice. n=6 C, 6 KO. g. Principal Component Analysis (PCA) plot of RNAseq from AclyBATiKO and Acly,FasnBATiKO male mouse and littermate controls. h. Heat map relative abundance of Immune Response genes in RNAseq of AclyBATiKO and Fasn,AclyBATiKO BAT. Panel G-H: light grey (C), orange bar (AclyBATiKO), dark grey (C), light purple bar (Fasn,AclyBATiKO). Data are mean ± s.e.m. Statistical analysis unpaired two-tailed Student’s t-test. Group differences determined via one-tailed ANOVA with Tukey’s post hoc.

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