Extended Data Fig. 9: Metabolites associated with YFAS.

a) Volcano plot of metabolites associated with YFAS identified employing robust linear regression models (t-statistic based on M-estimation with Huber weighting solved using Iteratively Reweighted Least Squares) in the IRONMET-CGM cohort adjusting for age, sex, BMI, years of education. b) Scatter-plots (two-sided partial Spearman’s rank correlation test adjusted for age, BMI, sex and education years) between 3-ethilmalic acid and YFAS residuals. Scatter-plots show tendency line with 95% confidence interval, each dote represent and independent participant. c) Dot-plot of KEGG enriched pathways (one-sided hypergeometric test) from metabolites significantly associated with YFAS in IRONMET-CGM cohort. P values were corrected for multiple testing using the Storey correction (q-values). d) Metabolite concept network depicting significant metabolites involved in the selected pathways. e) Dot plot of significantly over-represented (one-sided hypergeometric test) HMDB pathways identified from significantly differential metabolites associated with YFAS identified in the IRONMET-CGM. P values were corrected for multiple testing using the Storey correction (q-values). f) Metabolite concept network depicting significant metabolites involved in the selected pathways.