Extended Data Fig. 10: Experiments of food addiction in Drosophila.
a–c) Cumulative plot of the PI over time. The middle line represents the mean and the shading the S.E.M and Cumulative PI at 40 min of flies choosing between a 5% sucrose or 5% sucrose food + 15% ethanol. Flies expressing impTNT or TNT in the dopaminergic neurons by means of the ple-Gal4 promoter with and without dietary supplementation of AA (200 mg/L). PI ranging from −1 to +1, positive values indicate a preference for ethanol and negative values indicate preference for sucrose (PimpTNT-TNT < 0.0001). The graph represents data from a minimum of 3 independent experiments. Bar graphs represent mean with S.E.M. Significance was calculated using Mann–Whitney test (****p < 0.0001). d) Gene–gene interaction network (minimum confidence score > 0.15) constructed using differentially expressed mPFC genes (abs(logFC>1), pFDR<0.2) via the Search Tool for the Retrieval of Interacting Proteins/Genes (STRING) database. The network nodes are genes and the edges represent the predicted functional interactions. The thickness indicates the degree of confidence prediction of the interaction. e) Dot plot of GO BP, CC and MF over-representation analysis (one-sided hypergeometric test) of differentially expressed mPFC genes according to the presence or absence (<10 counts) of Gokushovirus WZ-2015a in the human donor’s microbiota. P values were corrected for multiple comparisons using the Storey correction (q-value). f) Gene-concept network depicting the connections of those genes involved in the GO biological process, cellular compartment and molecula function over-representation results. g) Gene-concept network depicting the connections of those genes involved in the KEGG-based over-representation results.
