Extended Data Fig. 8: Changes to the feeding schedule alter G6pdx expression and affect hepatocyte proliferation.
a-c, Immunoblotting showing the indicated protein levels in Hepa1-6 cells cultured in glucose-free medium (a), and in the livers from mice fasted for 2 days (b) or AAV-infected (c). d, Representative IHC images and quantification of Ki67-positive cells from mice with the indicated treatment. More than three fields for each of three independent samples were counted. Scale bar, 100 μm. e,f, The mRNA levels (e) and enzymatic activity (f) of G6pdx in the livers of AL- or 10-day IF- mice. g, qRT-PCR showing the expression of other PPP related genes in the livers of mice fed AL or IF. h, Quantification of the ratio of phosphorylated (p)-Bmal1 to total Bmal1 from the blots as shown in Fig. 6h. i, Immunoblotting showing the levels of the indicated proteins in chromatin fraction. j,k, Representative IHC images (j) and quantification (k) of Ki67-positive cells from mice with the indicated treatment. Five fields for each of three independent samples were counted. For e-h, n = 3 or 4 mice per group. Data are mean ± s.e.m. P-values were calculated using two-way ANOVA with Tukey’s test (d), unpaired two-tailed Student’s t-test (e,f,h) and multiple unpaired two-tailed Student’s t-test (g,k).
