Extended Data Fig. 7: Paraspeckle ameliorates hyperglycaemia by modulating hepatic gluconeogenesis in diabetic mice.
From: Proline exacerbates hepatic gluconeogenesis via paraspeckle-dependent mRNA retention
a, Neat1 level in the liver from WT or ob/ob mice, n = 6 mice. b, Representative images showing cellular localization of Neat1 (green) in the liver from WT or ob/ob mice. Nuclei are stained with DAPI (blue). c, Quantitation of fluorescence intensity of Neat1, n = 4 mice. d, mRNA levels of Sfpq, Nono, and Pspc1 in liver from WT or ob/ob mice, n = 6 mice. e, Representative immunoblots for SFPQ, NONO, and PSPC1 protein levels in liver from WT or ob/ob mice. α-Tubulin as a loading control. f, Total Ppargc1a and Foxo1 mRNA levels in liver from WT or ob/ob mice, n = 6 mice. g, Nuclear and cytoplasmic Ppargc1a and Foxo1 mRNA levels, n = 4 mice. h, Representative immunoblots for PGC1α and FOXO1 protein levels. α-Tubulin as a loading control. i, G6pc and Pck1 mRNA levels in WT and ob/ob mice, n = 4 mice. Data are presented as means ± SD. Statistical significance was determined using a two-tailed Student’s t test in (a), (c), (d), (f), (g), and (i).
