Extended Data Fig. 8: Neat1 overexpression ameliorates hyperglycaemia in obesity mice.
From: Proline exacerbates hepatic gluconeogenesis via paraspeckle-dependent mRNA retention
a, Water intake and food consumption of ob/ob (n = 5 per group) or db/db (n = 7 per group) mice, with and without Neat1 activation. b-, Body weight, n = 5 ob/ob mice per group; n = 7 db/db mice per group. c, Liver weight, n = 5 ob/ob mice per group; n = 7 db/db mice per group. d, Representative image of hematoxylin and eosin (H&E) staining of liver sections. e, Serum AST and ALT levels, n = 5 ob/ob mice per group; n = 7 db/db mice per group. f, Hemoglobin A1c (HbA1c) levels in whole blood, n = 5 ob/ob mice per group; n = 7 db/db mice per group. g,h, Ppargc1a, Foxo1 (g) Gpt and Gpt2 mRNA levels (h) in liver, n = 5 ob/ob mice per group; n = 7 db/db mice per group. i,j, G6PC and PCK1 protein levels (i) or enzymatic activity (j), n = 5 ob/ob mice per group; n = 7 db/db mice per group. k, Urine glucose levels, n = 7 mice per group. l, Random blood glucose levels, n = 7 mice per group. m, Insulin tolerance tests (ITT) after fasting and statistical analysis of AOC (Area of the curve; subtracting the baseline) for the ITT, n = 5 mice per group. n, Glucose tolerance tests (GTT) after fasting and statistical analysis of AOC for the GTT, n = 7 mice per group. Data are presented as means ± SD. Statistical significance was determined using a two-tailed Student’s t test in (a), (b), (c), (e), (f), (g), (h), (i), (j), (k), (l), (m), and (n).
