Extended Data Fig. 10: Paraspeckle ameliorates hyperglycaemia by modulating hepatic gluconeogenesis in female ob/ob mice.
From: Proline exacerbates hepatic gluconeogenesis via paraspeckle-dependent mRNA retention
a, Schematic illustration of the experimental design to evaluate the effect of Neat1 overexpression in ob/ob female mice, n=5 per group. b, Water intake and food consumption, n = 5 mice per group. c, Body weight, n = 5 mice per group. d, Liver weight, n = 5 mice per group. e, Representative image of hematoxylin and eosin (H&E) staining of liver sections. f, Serum AST and ALT level, n = 5 mice per group. g, HbA1c levels in whole blood, n = 5 mice per group. h, Neat1 RNA level in liver, n = 5 mice per group. i, Representative images showing cellular localization of Neat1 (green) in liver. DAPI staining (blue) marks the nucleus. Quantitation of fluorescence intensity of Neat1 (right panel). j,k, Total Ppargc1a and Foxo1 mRNA levels (j), Nuclear-to-cytoplasmic ratio of Ppargc1a and Foxo1 (k) in liver, n = 5 mice per group. l, Representative immunoblots for PGC1α and FOXO1 protein levels. α-Tubulin as a loading control. m,n, G6pc, Pck1 (m), Gpt, and Gpt2 (n) mRNA levels, n = 5 mice per group. o,p, G6PC and PCK1 protein levels (o) or enzymatic activity (p) in liver, n = 5 mice per group. q, PTT after fasting and statistical analysis of AUC for the PTT, n = 5 mice per group. r, Fasting blood glucose levels, n = 5 mice per group. s, Urine glucose levels, n = 5 mice per group. Data are presented as means ± SD. Statistical significance was determined using a two-tailed Student’s t test in (b), (c), (d), (f), (g), (h), (i), (j), (k), (m), (n), (o), (p), (q), (r), and (s).
