Extended Data Fig. 4: Anatomical and spectral characterization of epidermis and dermis of human skin samples.
From: Subcutaneous depth-selective spectral imaging with mμSORS enables noninvasive glucose monitoring
a, Bright field image of a processed ex-vivo human skin cross-section. A forearm cross-section from one woman of 29 years old were imaged and repeated independently five times with similar results, the zoom-in region of interest was shown. Epidermis is rich in cells, and thus, nucleic acid, while dermis is rich in collagen. The yellow dashed curve indicates the DEJ. Green dots indicate the distribution of glucose molecules, predominantly within the dermis. Scale bar: 250 µm. b, Reference Raman spectra taken from ex-vivo epidermis (black) and dermis (blue) samples of human skin. An ex-vivo fresh upper back tissue from one man of 28 years old was obtained and cut manually to prepare epidermal and dermal samples, five spectra were collected from different region of interest for each sample and the mean spectra were shown as reference spectra. Pink and purple shades indicate characteristic Raman peaks of nucleic acid and collagen.
