Extended Data Fig. 1: Information summary of cynomolgus monkeys and phenotypical analysis in young and old monkey skeletal muscles.
From: SIRT5 safeguards against primate skeletal muscle ageing via desuccinylation of TBK1
a. Information summary of the cynomolgus monkeys analyzed in this study. b.WGA staining in young and old monkey skeletal muscles. Left, representative images. Scale bars, 100 μm. Right, the percentages of central nuclei were quantified. Arrows indicate the central nuclei in skeletal muscles. c.The percentage of type IIX (MYH1-positive) fires in young and old monkey skeletal muscles was quantified. d, e. Immunofluorescence staining of LAP2 (d) and HP1γ (e) in young and old monkey skeletal muscles. Left, representative images. Scale bars, 100 μm and 25 μm (zoomed in). Right, the proportion of positively stained nuclei was quantified. Arrows indicate the positively stained nuclei in b, d and e. Two-tailed Student’s t-test was used for statistical analysis and data are shown as the mean ± s.e.m. n = 8 monkeys from both genders per group in b–e. P values are annotated in the figures.
