Extended Data Fig. 8: 3D distribution of representative lipids highlighting anatomical regions in the mouse brain.
From: AI-driven framework to map the brain metabolome in three dimensions
Representative images showing the spatial distribution of specific lipid species in mouse brain regions. (Left) Nissl-stained sagittal brain section of the same plane from the Allen Brain Atlas and Allen Reference Atlas, – Mouse Brain, Allen Mouse Brain Atlas, mouse.brain-map.org and atlas.brain-map.org with regions of interest highlighted in red, including the isocortex, hindbrain, cerebellar molecular layer, hippocampal region, ventricular system, and cerebral nuclei. (Middle) Sagittal plane images obtained using 2D MALDI imaging from our study, illustrating the relative abundance and localization of the indicated lipid species. (Right) 3D visualization of lipid distributions, highlighting the top 1% highest intensity pixels to resolve their spatial organization. Lipid species are annotated above each row: PE 38:6 [M-H]⁻, PE 36:2 [M-H]⁻, PS 44:12 [M-H]⁻, PE 38:4 [M-H]⁻, PI 40:4 [M-H]⁻, and LPE 20:4 [M-H]⁻. Intensity scales range from minimum (purple) to maximum (yellow/red) as depicted in the bottom scale bar.
