Extended Data Fig. 1: Validation of mitochondrial localization of NCLX-BioID2-HA fusion protein and TMEM65.
From: TMEM65 regulates and is required for NCLX-dependent mitochondrial calcium efflux
a, Western blots showing progressive digestion of mitochondria from AC16 cardiomyocytes expressing NCLX-BioID2HA with increasing concentrations of trypsin, +/− increasing concentrations of digitonin to disrupt the inner mitochondrial membrane. WCL, whole cell lysate; Cyto, cytosolic fraction. CypD, cyclophilin D. O.M.M., outer mitochondrial membrane; I.M.M., inner mitochondrial membrane. Data are representative of 3 independent experiments. b, Immunofluorescence staining of AC16 cardiomyocytes transiently transfected with a plasmid encoding TMEM65-Myc-FLAG or empty vector control, corresponding to experiment shown in Fig. 1f. Data are representative of 2 independent experiments.
