Extended Data Fig. 4: Validation of expression and mitochondrial localization for TMEM65 mutant constructs.
From: TMEM65 regulates and is required for NCLX-dependent mitochondrial calcium efflux
a, Sequence alignments of C-terminal Myc-FLAG-tagged wild-type (WT) and mutant human TMEM65 constructs encoded by TMEM65 adenoviruses, corresponding to schematic shown in Fig. 3k. (TM = transmembrane helix). b, c, Western blots of AC16 cardiomyocytes transduced with adenovirus encoding control LacZ, WT TMEM65-Myc-FLAG, or mutant TMEM65-Myc-FLAG. Data include 3 biological replicates/group. d, Immunofluorescence staining of AC16 cardiomyocytes transduced with adenovirus encoding control LacZ, WT TMEM65-Myc-FLAG, or mutant TMEM65-Myc-FLAG. TOM20 served as a mitochondrial marker. Images are representative of 2 independent replicates/group. Cterm., C-terminus.
