Fig. 7: Dietary effects on sleep and activity patterns are influenced by gut Tk signalling and AKH pathways in mated female flies.
From: Protein-responsive gut hormone tachykinin directs food choice and impacts lifespan
a, Left, sleep profile graphs showing the percentage of flies asleep over time for controls and gut-specific Tk-knockdown animals consuming sugar and yeast diets. The shaded area represents s.e.m. Right, quantification of sleep during the midday ‘siesta’ from Zeitgeber time (ZT) 4 to 8 h when flies normally exhibit high sleep, in animals with Tk knockdown in the gut on sugar and yeast diets (two-tailed Mann–Whitney U-tests). b,c, Left, sleep profiles of controls and animals with RNAi against TkR99D (b) or Akh (c) in APCs. Right, quantification of midday sleep in these animals (two-tailed Mann–Whitney U-tests). d,e, Left, sleep profiles of controls and animals with RNAi against AkhR in the fat body (d) or pan-neuronally (e). Right, quantification of midday sleep in these animals (two-tailed Mann–Whitney U-tests). f, A model of Tk function consistent with these data. Sample sizes (n) and P values are indicated in each plot. n represents animals per group. Dot plots indicate the median and 95% confidence interval. NS, P > 0.05.
