Extended Data Fig. 1: Effects of gut-specific Tk knockdown on feeding behavior and metabolic homeostasis in males and mated female flies.
From: Protein-responsive gut hormone tachykinin directs food choice and impacts lifespan
Comparing controls (Tkgut>) and gut-specific Tk knockdowns (Tkgut > Tk-RNAi). a, One-hour dye-feeding intake of sugar (left) and yeast (right) by male flies fed on a yeast diet for 15 h (two-tailed unpaired Student’s t test). b, One-hour dye-feeding sugar intake for UAS-Tk-RNAish alone (two-tailed Mann-Whitney U). c, Six-hour CAFÉ assay for sugar intake for UAS-Tk-RNAiKK alone (two-tailed Mann-Whitney U test). d, FLIC observation of sugar-directed feeding behavior over 22 h for UAS-Tk-RNAish alone (two-tailed unpaired Student’s t test). e and f, Whole-body triacylglyceride (e) and glycogen (f) levels measured under fed and 24-hour-starved conditions for controls and animals expressing EEC-specific RNAi against Tk (one-way ANOVA/Dunnett’s). g, Starvation-survival curves for controls and animals expressing EEC-specific RNAi targeting Tk (log-rank Mantel-Cox tests). Sample sizes (N) and p values are indicated in each plot. In a–d and g, N = animals per group. In e and b, N = whole-body samples. Box plots show the median, the bounds of the box (25th and 75th percentiles), and whiskers representing the minimum and maximum values. Panels d, e, and f indicate the mean ± SEM. ns, non-significant (p > 0.05).
