Extended Data Fig. 4: Related to NADK deletion alters subcellular contributions to 1 C production and depletes cellular folate pools.
From: Cytosolic NADK is conditionally essential for folate-dependent nucleotide synthesis
(a) Fractional labeling of dTTP in control cells (mean ± s.d., n = 3 biologically independent samples). Value above bracket indicates difference in M + 1 labeling. Two-tailed Welch’s t-test for comparisons of M + 1 labeling. (b) Immunoblot for expression of SHMT2 in control and SHMT2-knockout cells. RAPTOR served as the loading control. EV, empty vector. (c) Fractional labeling of ATP in SHMT2-knockout cells (mean ± s.d., n = 3 biologically independent samples). Values above bracket indicate differences in M + 1 (bottom bracket) and M + 2 (top bracket) labeling. Only unlabeled ATP could be detected from SHMT2-knockout cells transduced with empty vector (EV). Two-tailed Welch’s t-test for comparisons of M + 1 (bottom) and M + 2 (top) labeling.
