Extended Data Fig. 4: nSREBP2 undergoes phase separation through the N-terminal IDR. | Nature Metabolism

Extended Data Fig. 4: nSREBP2 undergoes phase separation through the N-terminal IDR.

From: Nuclear SREBP2 condensates regulate the transcriptional activation of lipogenic genes and cholesterol homeostasis

Extended Data Fig. 4

a, Amino acid composition analysis of human nSREBP2. Ticks represent amino acids indicated on the Y axis at the positions indicated on the X axis. b, Net charge per amino acid residue analysis of nSREBP2. c, Schematic of structural model of nSREBP2 by Alphafold2. d, Recovery curve of nSREBP2 IDR-N condensates in FRAP by optoDroplet system in Fig. 4h. Data were shown as mean ± s.d. (n = 3 independent biological replicates). e, Purified nSREBP2 IDR-N-mEGFP showing a concentration-dependent increase in turbidity at increasing levels of large polymeric crowders. f, Recovery curve of nSREBP2 IDR-N-mEGFP droplets in vitro in FRAP in Fig. 4k. Data were shown as mean ± s.d. (n = 3 independent biological replicates). g, In vitro droplet formation analysis of nSREBP2 IDR-N-mEGFP (7.5 μM) with the indicated concentrations of NaCl. Scale bar, 3 μm. Imaging data represents three independent experiments with similar results.

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