Extended Data Fig. 5: F178 is critical for nSREBP2 phase separation and transactivation of target genes. | Nature Metabolism

Extended Data Fig. 5: F178 is critical for nSREBP2 phase separation and transactivation of target genes.

From: Nuclear SREBP2 condensates regulate the transcriptional activation of lipogenic genes and cholesterol homeostasis

Extended Data Fig. 5

a, Representative live cell images of CHO-K1 nuclei expressing nSREBP2 PrLD2 truncation variants and point mutations with light-induced condensation by Corelet system. Scale bar, 3 μm. Imaging data represents three independent experiments with similar results. b, Representative live cell images of CHO-K1 nuclei expressing nSREBP2 PrLD2 truncation variants and point mutations with light-induced condensation by optoDroplet system. Scale bar, 3 μm. Imaging data represents three independent experiments with similar results. c, F178A mutation impaired the expression of SREBP2 target genes in HeLa (SREBP2 KO) cells (n = 3 independent biological replicates). Data were shown as mean ± s.d. Statistical significance was calculated with one-way ANOVA followed by Turkey’s multiple comparison. d, Western blotting showing that HMGCS1 PCP-GFP was expressed the same in overexpressed WT, PrLD2 deletion and F178A mutation in HeLa (SREBP2 KO) cells.

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