Extended Data Fig. 8: Effects of nSREBP2 SNP variants and F178Y on the formation of nuclear condensates.
a, Representative live cell images of CHO-K1 cell nuclei expressing nSREBP2 SNP variants with light-induced condensation by optoDroplet system. b, Schematic of nSREBP2 domains and the structural model of F178 and P183 residue by Alphafold2. c, Immunofluorescence analysis of nSREBP2 P183S in HeLa (SREBP2 KO) cells and quantification of the number of condensates per cell. n = 30 cells WT, 28 cells F178A and 29 cells P183S, the cells per component were captured from three different coverslips at random and the condensate numbers were analyzed with manual analysis by Image J. d, Effect of nSREBP2 P183S on the transcriptional activity of SREBP luciferase reporter (n = 3 independent biological replicates). e, Effect of nSREBP2 P183S on the expression of SREBP2 target genes (n = 3 independent biological replicates). f, Representative live cell images of CHO-K1 cell nuclei expressing nSREBP2 P183S mutation with light-induced condensation by optoDroplet. g, Sequence alignment of human nSREBP2 IDR-N and nSREBP1c IDR-N. h, Representative live cell images of CHO-K1 cell nuclei expressing nSREBP1c truncations with light-induced condensation by optoDroplet system. a,c,f,h, Scale bars for the images are 3 μm. Imaging data represents three independent experiments with similar results. c,d,e, Data were shown as mean ± s.d. Statistical significance was calculated with one-way ANOVA followed by Turkey’s multiple comparison.
