Table 2 Adipose cell-type annotations and proposed marker genes in human single-cell data

From: Towards a consensus atlas of human and mouse adipose tissue at single-cell resolution

Cell types

Human cell-type markers

Cell subtypes

Human cell-subtype markers

Adipocytes

ADIPOQ5,82,83,94,124,133,176,189,190, PLIN1 (refs. 124,176)

White

 

Brown/beige

COBL79, CKMT2 (ref. 79), PPARGC1A82, ATP5K136, COX7A1 (ref. 136)

ASPCs

PDGFRA5,83,94,98,124,134,190, DCN10,82,190

Stem cells/early preadipocytes

DPP4 (refs. 88,93,124,133), CD55 (refs. 88,93,124,133), PI16 (refs. 93,124,133,176)

Committed preadipocytes

PPARG88,93,124,176

Pericytes

STEAP4 (refs. 5,94,124,176,177), COL25A1 (refs. 124,176)

  

SMCs

MYOCD5,122,124,176, MYH11 (refs. 10,82,122,124,133,134,176,177,190)

  

ECs

PECAM1 (refs. 5,98,122,124,190), MECOM82,124,176,177, CDH5 (ref. 122), ENPEP5

Lymphatic ECs

PROX1 (refs. 94,124,134,176,189,191), LYVE1 (refs. 97,124,134,176,191)

Arterial ECs

PCSK5 (refs. 176,191), GJA5 (refs. 122,124,191), NEBL122,176,177,191

Venous ECs

ACKR1 (refs. 122,124,134,176,191)

Capillary ECs

BTNL9 (refs. 124,134,176,190,191, RBP7 (ref. 122), CA4 (refs. 122,191)

Mesothelial cells

MSLN5,83,94,124, KRT19 (ref. 5), BNC1 (ref. 124)

  

B cells

MS4A1 (refs. 5,83,94,124,134), CD79A97,98, IGKC98

  

Plasmablasts

JCHAIN124

  

T cells

CD3D82,134, CD3E82

Treg cells

FOXP3 (refs. 98,124), TIGIT98,124, CTLA4 (refs. 5,98,124)

(Naive) CD4+ T cells

CD2 (ref. 98), CD4 (ref. 134), CD40LG98,124

(Naive) CD8+ T cells

CD8A98,134, GZMK98,124

Cytotoxic CD8+ T cells

CD8A98,134, GZMB98,134, GZMH98

CD8+ γδ T cells

CD8A98, LEF1 (ref. 98), KLRC2 (ref. 98), ZNF683 (ref. 98)

Mucosal-associated invariant T cells

CD8A98, KLRB1 (ref. 98), GZMK98,124, SLC4A10 (ref. 124)

NK cells

GNLY10,97,98,124, KLRD1 (ref. 5), PRF1 (ref. 98)

Mature NK cells

FCGR3A98,124, FGFBP2 (ref. 98), PRF1 (ref. 98), KLRF1 (ref. 98)

Tissue-resident NK cells (NK-like)

XCL2 (ref. 98), GZMK98, KLRC1 (ref. 98)

Immature NK cells (NK-like)

XCL2 (ref. 98), GZMK98, KLRC1 (ref. 98), NCAM1 (ref. 98), CD2 (ref. 98), IL7R98, SELL98

ILCs

IL7R98, CD200R1 (ref. 98)

ILC1

ZNF683 (ref. 98), TBX21 (ref. 98), CD200R1 (ref. 98), IL7R98

ILC2

GATA3 (ref. 98), IL1RL1 (ref. 98), CD200R1 (ref. 98), IL7R98

ILC3

CCR6, IL1R1 (ref. 98), IL23R98, CD200R1 (ref. 98), IL7R98

ILCP

SELL98, IL1R1 (ref. 98), CD200R1 (ref. 98), IL7R98

DCs

FLT3 (refs. 5,82,98)

cDC1

XCR1 (refs. 98,192), CLEC9A98,124,192, CADM1 (refs. 98,124,192), DPP4 (refs. 98,192)

Activated DCs

CCR7 (refs. 98,124,193), FSCN1 (ref. 98), CCL22 (ref. 98), LAMP3

cDC2B

FCER1A98,124,134,192, CD1C98,124,134,192, CLEC10A98,124,176,192, IRF4 (refs. 98,192)

Neutrophils

S100A12 (ref. 98), S100A9 (ref. 98), CSF3R5,124

  

Monocytes

FCN1 (refs. 124,192)

Classical monocytes 1

FCN1 (refs. 98,124), FCER1A98, TXNIP98

Classical monocytes 2

FCN1 (refs. 98,124), FCAR98

Non-classical monocytes

FCGR3A98,124,134,192, HES4 (refs. 98,124,192), LST1 (refs. 98,134), DGKG5

Macrophages

MRC1 (refs. 134,136,176,177,192), F13A1 (refs. 124,136,176)

PVMs

LYVE1 (refs. 98,124,134,176), SELENOP98,176,192, C1QA10,98

LAMs

CD9 (refs. 97,98,124,134), TREM2 (refs. 98,124,134,176), LPL97,98,124,176

Inflammatory macrophages

CCL3L1 (ref. 98), TNF98, CXCL3 (refs. 97,98)

Mast cells

KIT83,124,176,190, CPA3 (refs. 5,94,124,134,176,177)

  

Schwann cells

XKR4 (ref. 124)

  
  1. A summary of commonly used cell-type and subtype marker genes reported in human scRNA-seq/snRNA-seq studies to date. Distinguishing closely related cellular subtypes often requires a combination of markers. Moreover, many subtype markers do not necessarily differentiate subtypes from other cell types within the tissue and are therefore typically useful only for distinguishing subtypes within a specific cell type or a small subset of related types. cDC, conventional dendritic cell.
Back to article page