Extended Data Fig. 6: Electrophysiological characterization of PVNGLP-1R→DVC neurons under different energy states.
From: State-dependent central synaptic regulation by GLP-1 is essential for energy homeostasis
a. Experimental paradigm. b. Intrinsic electrophysiological characterization of PVNGLP-1R→DVC neurons. Summary of capacitance (two-tailed t-test, t(52) = 0.6357, p = 0.5279; Fed n = 24 cells/3 mice, Fasted n = 28 cells/3 mice). c. Input resistance (two-tailed Mann-Whitney test, p = 0.7134; Fed n = 24 cells/3 mice, Fasted n = 28 cells/3 mice). d. Resting membrane potential (two-tailed Mann-Whitney test, p = 0.0049; Fed n = 24 cells/3 mice, Fasted n = 28 cells/3 mice). e. Representative traces of spontaneous action potentials (sAPs). f. Quantification of sAP frequency (two-tailed Mann-Whitney test, p = 0.3148; Fed n = 24 cells/3 mice, Fasted n = 28 cells/3 mice). g. Representative traces of neurons responding to ramping current injection. Insert show the current injection protocol. h. Quantification of ramping current injection action potential firing number (two-tailed Mann-Whitney test, p = 0.3461; Fed n = 25 cells/3 mice, Fasted n = 27 cells/3 mice). i, Representative traces of neurons in response to stepped current injection. Insert shows the current injection protocol. j. Plot of the number of APs as a function of injected current (two-way ANOVA, Group effect: F(1, 48) = 0.7961, p = 0.3767; Time effect: F(1.340, 64.33) = 98.47, p < 0.0001; interaction: F(5, 240) = 0.3641, p = 0.8728; Fed n = 24 cells/3 mice, Fasted n = 26 cells/3 mice). Data are presented as mean ± SEM. ∗∗p < 0.01.
