Extended Data Fig. 7: Electrophysiological characterization of PVNGLP-1R→DVC neurons in HFD-induced obesity animals.
From: State-dependent central synaptic regulation by GLP-1 is essential for energy homeostasis
a. Experimental paradigm. b. Body weight of control and HFD-induced obese animals (two-tailed t-test, t (11) = 4.671, p = 0.0012; control n = 5 mice, HFD n = 6 mice). c. Intrinsic electrophysiological characterization of PVNGLP-1R→DVC neurons. Summary of capacitance (c) (two-tailed Mann-Whitney test, p = 0.2638; control n = 20 cells/3 mice, HFD n = 32 cells/3 mice). d. Resting membrane potential (RMP) (two-tailed t-test, t (51) = 3.212, p = 0.0023; control n = 20 cells/3 mice, HFD n = 31 cells/3 mice). e. Representative traces of spontaneous action potentials (sAPs). f. Pooled data of sAP frequency (two-tailed t-test, t (20) = 2.042, p = 0.0561; control n = 6 cells/3 mice, HFD n = 14 cells/3 mice). g. Representative traces of neurons in response to ramp current injection. Inset shows the current injection protocol. h. Quantification plot of ramping current injection-induced AP firing number (two-tailed Mann-Whitney test, p = 0.5784; control n = 20 cells/3 mice, HFD n = 30 cells/3 mice). i. Representative traces of neurons in response to stepped current injection. Inset shows the current injection protocol. j. Plot of the number of APs as a function of injected current (two-way ANOVA, Group effect: F(1, 48) = 0.1073, p = 0.7447; Time effect: F(1.362, 64.97) = 157.7, p < 0.0001; interaction: F(10, 477) = 0.7476, p = 0.6795; control n = 20 cells/3 mice, HFD n = 30 cells/3 mice). Data are presented as mean ± SEM. ∗∗p < 0.01.
