Extended Data Fig. 10: Comprehensive metabolic analyses of animals after inactivation of PVNGLP-1R→DVC synaptic release and daily food consumption.
From: State-dependent central synaptic regulation by GLP-1 is essential for energy homeostasis
a. Energy expenditure (EE) (two-way ANOVA, the main effect of Group: F(1, 14) = 0.01004, p = 0.9216; main effect of Time: F(6.449, 90.29) = 4.545, p = 0.0003; interaction between Group and Time: F(159, 2226) = 1.054, p = 0.3132; control n = 7 mice, TeNT n = 9 mice). b. The volume of carbon dioxide produced (VCO2) (two-way ANOVA, the main effect of Group: F(1, 14) = 0.1053, p = 0.7503; main effect of Time: F (6.291, 88.08) = 4.765, p = 0.0002; interaction between Group and Time: F(159, 2226) = 0.9634, p = 0.6126; control n = 7 mice, TeNT n = 9 mice). c. Respiratory exchange ratio (RER) (two-way ANOVA, Group effect: F (1, 14) = 4.844, p = 0.0450; Time effect: F(3.463, 48.49) = 2.22, p = 0.0893; interaction: F (159, 2226) = 0.442, p > 0.9999; control n = 7 mice, TeNT n = 9 mice). d. Oxygen consumed (VO2) (two-way ANOVA, Group effect: F(1, 14) = 0.003172, p = 0.9559; Time effect: F(6.303, 88.25) = 3.659, p = 0.0023; interaction: F(119, 1666) = 1.157, p = 0.1256; control n = 7 mice, TeNT n = 9 mice). e. Average locomotor activity of TeNT and control (GFP) animals (two-way ANOVA, Group effect: F(1, 12) = 1.862, p = 0.1974; Time effect: F(8.698, 104.4) = 2.654, p = 0.0089; interaction between Group and Time: F(159, 1908) = 1.101, p = 0.1919; control n = 6 mice, TeNT n = 8 mice). f. Experimental paradigm for inactivating PVNGLP-1R→DVC synaptic release. Cre-dependent expression of Flpo and Flpo-dependent expression of TeNT in PVNGLP-1R →DVC neurons. g. Average daily food intake of EYPF HFD-fed obese animals’ baseline for 2 days and after i.p. injection of 200 μg/kg liraglutide for 5 days (two-tailed paired t-test, t(7) = 4.658, p = 0.0035; EYFP n = 7 mice). h. Average daily food intake consumption of TeNT HFD-fed obese animals’ baseline for 2 days and after i.p. injection of 200 μg/kg liraglutide for 5 days (two-tailed paired t-test, t(7) = 5.595, p = 0.0014; TeNT n = 7 mice). Data are presented as mean ± SEM. ∗∗p < 0.01.
