Extended Data Fig. 1: PVNGLP-1R neurons project to different downstream targets.
From: State-dependent central synaptic regulation by GLP-1 is essential for energy homeostasis
a. Experimental paradigm for tracing PVNGLP-1R neuronal outputs using AAV-mediated Cre-dependent expression of SynaptoTag2. The bicistronic expression cassette expresses cell-filling mCherry and a synapse-specific EGFP-synaptobrevin-2 fusion protein (EGFP-Syb2). b. Representative images of the PVN and downstream targets of PVNGLP-1R neurons in the brain of GLP-1R-ires-Cre mice with AAV-SynaptoTag2 injected into the PVN. c. Quantifications of downstream targets of PVNGLP-1R neurons in the brain. EGFP-Syb2 signal is normalized to the fluorescence intensity of the PVH (n = 4 mice). d. Experimental paradigm for retrograde tracing of DVC inputs from the GLP-1R expressing neurons. AAVrg-DIO-EYFP was injected into the DVC in GLP-1R-ires-Cre mice. e. Representative image of retrogradely labeled PVNGLP-1R →DVC neurons. f. Quantification of PVNGLP-1R→DVC neurons along the anterior to posterior axis (n = 3 mice). Data are presented as mean ± SEM. 3 V: the third ventricle; cc: central canal; scp: superior cerebellar peduncle.
