Extended Data Fig. 8: Dietary n-6 PUFA restriction or Cidec–/– mirrors Lpcat3AKO adaptation to DIO.
From: Dietary control of peripheral adipose storage capacity through membrane lipid remodelling
(a) Heatmap of the top 70 differentially expressed genes (35 up or down) in iWAT of 18-week-old control and Lpcat3AKO mice fed the indicated HFDs for 10 weeks (n = 4/group). (b,c) GSEA analyses of the top 500 upregulated genes in (b) iWAT of HFDn-3 or HFDsfa-fed control mice vs. HFD-fed controls or (c) in eWAT of ob/ob Cidec–/– mice vs. ob/ob controls75. The ranked list metric is the product of log2 (fold-change)* –log10 (adjusted P value) from the differentially expressed genes in iWAT from HFD-fed Lpcat3AKO mice vs. controls (NES). (d) Correlation matrix depicting the association between Lpcat3AKO markers and clinical traits in METSIM95. Node colour and size reflect the correlation directionality and p value. Correlations were analysed by midweight bicorrelation coefficient or corrected p value using the R package WGCNA. (e) Body weight (BW) gain curves and (f) composition of 28-week-old control and Lpcat3AKO female mice fed HFD for 20 weeks (n = 14, 13). (g) Wet weight of the indicated tissues in HFD-fed control and Lpcat3AKO female mice (n = 14, 13). (h) Liver wet weights (% of BW; n = 14, 13). Data are presented as mean ± SEM. ***P < 0.001 by two-way RM ANOVA with Sidak’s multiple comparisons test (e); Welch’s t-tests with FDR correction using the Benjamini, Krieger, and Yekutieli procedure (f,g); or Welch’s t-test (h).
