Fig. 3: NAA from adipose tissue regulates postprandial body temperature.
From: N-acetylaspartate from fat cells regulates postprandial body temperature
a,b, Body temperature (temp) of male (a) and female (b) Aspafl/fl and AspafKO mice following fasting (n = 23 Aspafl/fl, 29 AspafKO males; n = 8 Aspafl/fl, 9 AspafKO females) and refeeding (n = 23 Aspafl/fl, 29 AspafKO males; n = 8 Aspafl/fl, 9 AspafKO females). For the change in body temperature (ΔTemp), in paired males (n = 23 Aspafl/fl, 29 AspafKO) and females (n = 8 Aspafl/fl, 9 AspafKO), data are mean ± s.e.m., and *P < 0.05 by an unpaired two-tailed Student’s t-test. c, Plasma leptin levels in male mice following fasting (n = 22 Aspafl/fl, 23 AspafKO) and refeeding (n = 18 Aspafl/fl, 22 AspafKO). d,e, Relative abundance of NAA and pyrimidine metabolites in the scWAT (d) and plasma (e) of Aspafl/fl and AspafKO mice in fasted and refed states measured by IC–MS analysis (n = 4 mice per group per condition) and shown as log10-transformed values normalized to Aspafl/fl fasted levels. f, Hyperinsulinaemic–euglycaemic clamp (n = 5 per group). Glucose infusion rate (GIR), glucose production rate (GPR) during basal (empty bars) and clamp (striped bars) conditions, glucose disposal rate (GDR), and glucose uptake in vWAT and gastrocnemius muscle. Data are mean ± s.e.m. In a–e, data are represented as box-and-whisker plots using the Min-to-Max method in GraphPad Prism: box limits, 25th to 75th percentiles; centre line, median; whiskers, minimum and maximum values. For a–e, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001 by an ordinary two-way ANOVA followed by Fisher least significant difference (LSD) tests. For f, *P < 0.05 by an unpaired Student’s t-test (glucose infusion rate, glucose disposal rate and glucose uptake). Glucose production was not different after an ordinary two-way ANOVA.
