Extended Data Fig. 2: AspaKO mice display decreased glucose tolerance and plasma leptin levels while maintaining insulin sensitivity.
From: N-acetylaspartate from fat cells regulates postprandial body temperature
AspaWT and AspaKO mice were fed NCD for 12 weeks. a, Body weight gain of AspaWT and AspaKO mice over 12 weeks starting at 6 weeks of age (n = 6 WT,10 KO mice/group), shown as a percentage of initial body weight. Data represent mean ± s.e.m. ***P < 0.001 by ordinary two-way ANOVA. b, Glucose tolerance test (GTT) (n = 11 WT,9 KO mice/group) and (c) insulin tolerance test (ITT) (n = 10 WT,9 KO mice/group) with corresponding area-under-curve (AUC) measurements. Data represent mean ± s.e.m. *P < 0.05, **P < 0.01 by mixed-effects model followed by Sidak’s multiple comparisons test. AUC: **P < 0.01 by unpaired two-tailed Student’s t-test. d, Plasma insulin levels following 4 h fast (n = 10 WT,9 KO mice/group). Statistical analysis by unpaired two-tailed Student’s t-test. e, Representative histological analysis by H&E of ad-libitum fed liver across 3-5 fields of view (n = 4 mice/group); scale bars, 100μm. f, Ad-libitum fed liver triglyceride levels (n = 5,4 mice/group). Statistical analysis by unpaired two-tailed Student’s t-test. g, Mice were individually housed and monitored in CLAMS cages during a 96 h period with measurements of energy expenditure (EE). Data represent mean ± s.e.m. Statistical analysis was performed within CalR by ANCOVA with lean body mass as a covariate (n = 7 WT,6 KO mice/group). h, Representative histological analysis by H&E of ad-libitum fed BAT across 3-5 fields of view (n = 4 mice/group); scale bars, 100μm. i, BAT depot tissue weight, shown as a percentage of body weight (n = 11 WT,9 KO mice/group). b-d,f,i Data represented as box-and-whisker plots using the Min-to-Max method in GraphPad Prism: box limits, 25th to 75th percentiles; center line, median; whiskers, minimum and maximum values.
