Extended Data Fig. 3: Broad metabolic pathway alterations occur in WAT from AspaKO mice.
From: N-acetylaspartate from fat cells regulates postprandial body temperature
AspaWT and AspaKO mice were fed NCD for 12 weeks and tissues were used for reverse-phase protein array (RPPA) analysis of (a) scWAT and (b) vWAT from AspaWT and AspaKO mice. Relative abundance of (c) AMP and (d) GMP in scWAT and vWAT of AspaWT and AspaKO mice, measured by IC/MS targeted analysis (n = 4 mice/group). Data are mean ± s.e.m. e, Ratio of NAD/NADH across scWAT (n = 8 WT,6 KO mice/group) and vWAT (n = 8 WT,9 KO mice/group). Data are mean ± s.e.m. a-b Data shown as z-score of log2 transformed values and relative changes denoted in the colour bar (n = 4 mice/group). a-e #P < 0.1, *P < 0.05 by unpaired two-tailed Student’s t-test.
