Extended Data Fig. 2: Mouse NK cell activation is driven by IL-15.
From: Species-specific serine metabolism differentially controls natural killer cell functions
(a) PCA analysis of metabolites from naïve, 50 ng/mL IL-15-activated, or 50 ng/mL IL-15 and 200 IU/mL IL-2-activated mouse NK cells. (b) MFI of KLRG1, NKG2D, and CD69 of naïve, IL-15, or IL-2/15-activated mouse NK cells after 3 days activation. (c) Percent IFN-γ+ mouse NK cells stimulated with 50 ng/mL IL-15, 50 ng/mL IL-15 and 200 IU/mL IL-2, or 20 ng/mL IL-15 and 200 IU/mL IL-2. (d) MFI of phosphorylated STAT5 of mouse NK cells stimulated with 50 ng/mL IL-15 or 50 ng/mL IL-15 and 200 IU/mL IL-2. (e) Gating strategy for mouse NK cells. Data represent mean ± SEM of two independent experiments. Data representative of (a) n = 3, (b) n = 7 (naïve), n = 3 (IL-2/15), and n = 4 (IL-15), and (c, d) n = 3 (IL-2/15) and n = 4 (IL-15) independent mice. * p < 0.05, *** p < 0.001, **** p < 0.0001 by (b, c) two-way ANOVA or (d) two-sided Student’s t-test. Exact p-values: (b) (left to right) p = 0.0002, p < 0.0001, p < 0.0001; (c) p = 0.0173 IL-2 200 IU/mL + IL-15 20 ng/mL vs IL-2 200 IU/mL + Il-15 50 ng/mL, p = 0.0002 IL-2 200 IU/mL + IL-15 20 ng/mL vs IL-15 50 ng/mL.
