Extended Data Fig. 4: Enhanced ketogenesis during lactation promotes mitochondrial biogenesis and Ucp1 expression in iWAT.
a. Serum βHB concentrations in 3-week-old C57BL/6 J male mice orally administrated water or 1,3-butanediol (1,3BD; 5 g/kg BW) (n = 3/group). b. Serum βHB levels in P14 mice orally administrated water or 1,3BD starting at P2 (n = 4, 6). c. Intracellular βHB concentrations in P14 mice described in (b) (n = 4, 6). d. Body weight of mice from Fig. 3a (n = 4/group). e. iWAT weight of mice from Fig. 3a (n = 4/group). f. Dio2 and Elovl3 mRNA expression in iWAT of mice from Fig. 3a (n = 4/group). g. Adrb1, Adrb2 and Adrb3 mRNA expression in iWAT of mice from Fig. 3a (n = 4/group). h. Gross morphology of iBAT from mice in Fig. 3a. i. iBAT weight of mice from Fig. 3a (n = 4/group). j. Ucp1 mRNA expression in iWAT and iBAT of mice from Fig. 3a (n = 5, 4, 3, 3). k. Dio2 and Elovl3 mRNA expression in iBAT of control, Hmgcs2 KO, and 1,3BD-treated mice (n = 4, 4, 3). l. Temporal changes and quantification of oxygen consumption rate (VO2) in mice from Fig. 3m, housed at thermoneutrality (30 °C) and cold exposure (4 °C) (n = 5/group). m. Temporal changes and quantification of carbon dioxide production rate (VCO2) in mice from Fig. 3m, housed at 30 °C and 4 °C (n = 5/group). Data are expressed as means ± SD. Statistical analyses: two-sided Student’s t-test (a–c, g, j, l–m), one-way ANOVA with Tukey’s post hoc test (k) and two-way ANOVA with Tukey’s post hoc test for multiple comparisons (d–f, i).
