Extended Data Fig. 6: Optimization of inoculation delay in delayed co-culture of the Bn1 and Bn-BnAc3 strains (part 2).
From: A microbial process for the production of benzyl acetate
a, Two-phase extractive fermentation profile of delayed co-culture of the Bn1 and Bn-BnAc3 strains (n = 1). The Bn-BnAc3 strain inoculum (200 mL; OD600 = 7.8) was added after 72 h of fermentation. b, Apparent average biosynthesis rates of l-phenylalanine (Phe), trans-cinnamic acid (Cin), cinnamyl acetate (CinAc), benzoic acid (Bn), benzaldehyde (BnAl), benzyl alcohol (BnOH) and benzyl acetate (BnAc), calculated based on the changes in titres (panel a) and the molar masses of the compounds. c, Two-phase extractive fermentation profile of delayed co-culture of the Bn1 and Bn-BnAc3 strains (n = 1). The Bn-BnAc3 strain inoculum (200 mL; OD600 = 9.1) was added after 96 h of fermentation. d, Apparent average biosynthesis rates of benzyl acetate, cinnamyl acetate and intermediates between sampling points were calculated based on the changes in the titres (panel c) and the molar masses of the compounds. Symbols represent raw data points and bars represent apparent average biosynthesis rates.
