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CellPhoneDB combines a curated database of receptor–ligand interactions with methods to infer cell–cell communication from single-cell expression data. This protocol update describes an expanded database and new methods to infer, prioritize and visualize the results.
Mass spectrometry-based proteomic studies involve tissue lysis. Herein, we updated our 2018 protocol1 by implementing a sonication step into urea-based tissue lysis to improve the detection of membrane-bound and DNA-binding proteins.
