Alternative splicing generates diverse protein isoforms, yet the functions of most exons remain unknown. Here, the authors introduce scCHyMErA-Seq, a scalable single-cell CRISPR exon-deletion platform that maps exon-specific transcriptional functions shaping gene expression and cell-cycle states.
- Bandana Kumari
- Arun Prasath Damodaran
- Thomas Gonatopoulos-Pournatzis
