In this study a microscope is constructed that carries out simultaneous nonlinear absorption, Raman, and fluorescence (SNARF). Machine learning is then used to predict capillary-lining cell identities with 90% accuracy based on nuclear morphology and capillary relationship, which in combination with the developed microscope, can provide a means to study several fields such as neurovascular coupling.
- Andrew T. Francis
- Bryce Manifold
- Dan Fu